Profiling Metabolite Changes Within The Neuronal...

Profiling metabolite changes in the neuronal differentiation of human striatal neural stem cells using 1H-Magnetic Resonance Spectroscopy Yuen-Li Chung1, Gehan El Akabawy2,3, Po-Wah So4, Bhavana S. Solanky2,*, Martin O. Leach1, and Michel Modo2,5 1The Institute of Cancer Research and Royal Marsden Hospital, Cancer Research UK and EPSRC Cancer Imaging Centre, Sutton, Surrey, SM2 5PT, UK 2Kings College London, Institute of Psychiatry, Department of Neuroscience, London, SE5 9NU, UK 3University of Menoufia, Department of Anatomy and Embryology, Faculty of Medicine, Menoufia, Egypt 4Kings College London, Institute of Psychiatry, Department of Neuroimaging, London, SE5 9NU, UK 5University of Pittsburgh, Department of Radiology, McGowan Institute for Regenerative Medicine, Pittsburgh PA5203, USA Abstract Objective—Neural stem cells (NSCs) are playing an increasing clinical role for stroke. However at present, it is not yet possible to non-invasively monitor their differentiation once implanted into the brain. Methods—We here describe the use of high-resolution 1H-magnetic resonance spectroscopy (MRS) to define a metabolite profile of undifferentiated human striatal NSCs from the STROC05 cell line and their differentiation after 3 weeks of treatment with purmorphamine. Results—The undifferentiated conditions were characterized by ∠¼95% of cells expressing nestin and ∠¼77% being Ki67+, indicating that these were still proliferating. Phosphophocholine +glycerophosphocholine (PC+GPC)